Figure 7
Effect of disorganized DRM on Aβ degradation mediated by IDE. A- Bars showed percentage of degraded [125I]-Aβ by IDE immunoprecipitated from DRM and DSM fractions from membranes treated or not with MβCD (n = 2; *p < 0.05). B- Bars showed the semi-quantitative analysis of the percentage of degraded extracellular [125I]-Aβ in the conditioned media of control, MβCD treated and Chol replenished N2aWT cells (n = 6; *p < 0.05). C- Upper panel, representative western blotting of C-terminal APP and tubulin levels from N2aSW cellular homogenates showed no differences between control (CTL) and MβCD-treated cells. Lower panel, bars represented Aβ 40 levels in the supernatants of CTL and MβCD-treated cells after 12 and 24 hours of incubation (n = 2; *p < 0.05).