Changes in γ-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1

Table 1 Transient transfection of various PS1 constructs in HEK-293 cells derived stable cell lines expressing APP carrying Swedish mutation (H125.3-16).

Cell Line	Transfected PS1 Constructs	% change Aβ1–40	% change Aβ1–42	ratio of Aβ42/Aβ
H125.3-16	pcDNA	-	-	0.065 ± 0.004
"	PS1	-5.4	-8.6	0.063 ± 0.004
"	PS1-M146V	-10.5	33.7	0.095 ± 0.004**
"	PS1-D257TG	-5.4	-3.6	0.060 ± 0.003
"	PS1-D385TG	-2.7	233.7	0.193 ± 0.008*
"	PS1-D257TG/D385TG	-7.1	148.5	0.156 ± 0.001*

The levels of Aβ 40 and 42 were measured 48 hours post transfection.
** p < 0.05 as compared to PS1 wt transfected cells N = 3
* p < 0.01 as compared to PS1 wt transfected cells
Note: Due to the variation of absolute Aβ numbers in different experiments, only the comparative result to the cells transfected with pcDNA was used for comparison.

ISSN: 1750-1326