Immunofluorescence staining of APP and Aβ increases with 27-OHC. Immunostaining for APP and Aβ showed a decreased immunoreactivity to 6E10 antibody (green) in cells treated with 24-OHC compared treatment with 27-OHC. The immunoreactivity for 6E10 antibody in cells treated with a mixture of 24-OHC + 27-OHC is similar to that observed in control cells. DAPI (blue) was used as a nuclear counterstain. Bar 20 μm.