DJ-1 is detected in neurons from briefly fixed post mortem AD brains. A. Affinity purified antibody DJ-1-N detected neurons in brain section from AD cases. Staining was observed in pyramidal neurons of CA1, CA2 and CA3 subregions. B. Absence of staining in AD brain with anti-DJ-1-N preabsorbed with DJ-1 peptide, compared to an adjacent section immunostained with anti-DJ-N (A). C. DJ-1 immunoreactivity in a briefly fixed (~2 hours) section was easily detected. D. DJ-1-N failed to stain a routinely fixed (~2–4 weeks) section from the same AD case. E-G, Co-Staining of DJ-1 and GFAP in astrocytes of human brain sections. A routinely fixed section from control brain was stained with antibody DJ-1-N (E) and GFAP (F). Double immunofluorescent overlay indicates the location of DJ-1 in astrocytes (G). Magnification: A, B = 40×; C, D = 20×.