Redistribution of Gαi subunits after overexpressing calnuc-GFP. Gαi3 is found on the PM (arrows) and on the Golgi (G) in parental AtT-20 cells (A) and in those expressing calnuc-GFP (B). Some Gαi3 is found on RSG in cells expressing calnuc-GFP (B). Gαi1/2 is found mainly on RSG (arrows) in the cell bodies and tips of the cell processes (arrowheads) in both parental (C) and calnuc-GFP cells (D). More Gαi1/2 is observed on the PM (arrows) in cells overexpressing calnuc-GFP (D) cells than in parental AtT-20 cells (D).). Bar = 10 μm.