Cell cycle reactivation and cell death induction in LRRK2 deficient hmNPCs. Human mesencephalic neuroprogenitors were nucleofected with corresponding siRNAs (scrambled or LRRK2 siRNA) and differentiated for 2 weeks after a 24 h recovery period. Protein extracts were subjected to antibody microarray (Signal.screen 44 F employing 44 antibodies belonging to signalling cascades: MAPK, Akt/PKB, GSK, cell cycle regulation, apoptosis and transcription factors). Here we illustrate the comparison of sample 7 (LRRK2 siRNA) vs. sample 8 (scrambled siRNA): (A) the original spot intensities, (B) the differences and (C) selected averages presented in arbitrary units (a. u.). The differences in the intensities lower 10 a.u. should be neglected since they lay outside the test reliability.