Pull down experiment of brain homogenate. (A) Brain homogenates were prepared and pull-down experiments were done and studied by Western Blot. The results for Grb2 and Crk confirmed those found through in-vitro pull down. Pin1 results show that binding is increased through the phosphorylation of Thr-668. (B) Percent binding of the AID, ALID1, ALID2 and the phosphorylated ones to the brain homogenates protein: Grb2, Crk, Pin1. The interaction that has the greatest binding affinity was designated 100% and the others were represented as a fraction of the maximum binding. All percentages were rounded to the closest whole number.