Figure 1From: Insulin-degrading enzyme is exported via an unconventional protein secretion pathwayIDE secretion is unaffected by treatments that block the classical secretion pathway. A, Representative western blots of concentrated conditioned medium (CM) and cell lysates from immortalized hepatocytes co-expressing IDE and AAT and treated with classical secretion inhibitors BFA (20 μM), monensin (Mon; 50 μM) or nocodazole (Noc; 3.3 μM) or vehicle only (CTL). Note that the mature, secreted form of AAT migrates at 62 kDa. B, Quantification of western blots of secreted IDE and AAT. Data are mean ± SEM from 3 independent experiments.Back to article page