IDE secretion is unaffected by treatments that block the classical secretion pathway. A, Representative western blots of concentrated conditioned medium (CM) and cell lysates from immortalized hepatocytes co-expressing IDE and AAT and treated with classical secretion inhibitors BFA (20 μM), monensin (Mon; 50 μM) or nocodazole (Noc; 3.3 μM) or vehicle only (CTL). Note that the mature, secreted form of AAT migrates at 62 kDa. B, Quantification of western blots of secreted IDE and AAT. Data are mean ± SEM from 3 independent experiments.