PEA pretreatment protects HT22 cells from tBHP exposure. (A, B), HT22 cells were pretreated with 100 μM PEA for 5 - 6 hours prior to an overnight (10 - 12 hour or 16 - 20 hour) tBHP exposure. PEA pretreatment led to a significant neuroprotection against oxidative stress as determined by an increase in calcein fluorescence (A), and a reduction in fluorescent product from G-6-PD in the cell culture media (B). For this study, n equals three (3) experiments. HT22 cells were pretreated with PEA for 1 - 2 hours (C, D) or 12 hours (E, F) prior to tBHP exposure. PEA pre-treatment for 1 - 2 hours had no effect on viability (E), whereas pretreatment for 12 hours reduced measured extracellular G-6-PD activity (F). A P-value of ≤ 0.05, ≤ 0.01 and ≤ 0.001 is indicated by *, ** and ***, respectively, as determined by a Newman-Keuls multiple comparison test.