PEA treatment of HT22 cells leads to an increase in nuclear pAkt immunoreactivity. (A), a four (4) hour PEA treatment led to a significant decrease in Akt immunoreactivity but it had no effect on nuclear Akt immunoreactivity. For the treatment groups, n equals 50 and 66 cells for vehicle and PEA treatments, respectively. (B), a four (4) hour PEA treatment led to a significant increase in nuclear pAkt (red arrow) and a significant decrease in cytosolic pAkt. For the treatment groups, n equals 56 and 75 cells, respectively. A P-value of <0.05 and ≤ 0.01 is indicated by * and **, respectively, as determined by a two-sample t-test. The white scale bar is 25 μm.