Detection of inclusion bodies. Representative photomicrographs showing H & E staining (A and B), α-synuclein staining (C & D) and ubiquitin staining (E and F) in the two cell types (SH-SY5Y and CHME-5) collected at the end of 4 week's of rotenone (5 nm) treatment. SH-SY5Y cells showed eosinophilic (A, arrow head), α-synuclein positive (C, arrow head) and ubiquitin positive (E, arrow head) inclusion bodies. No inclusion bodies were seen in human microglial CHME-5 cells (B, D and F). Results shown are representative of three independent experiments. Scale bar: 50 μm.