Figure 2
From: ApoE mimetic peptide decreases Aβ production in vitro and in vivo
ApoE-derived peptide regulates APP trafficking in COS7 cells. COS7 cells were transiently transfected with APP and treated with control or apoE monomer (n = 3, A), apoE dimer (n = 3, C), or apoE trimer (n = 3, E) for 24 h. Total APP and β-actin levels in cell lysates were measured for loading control (lower panels). Quantification of biotin-labeled cell surface APP in COS7 cells showed a 12% (B), 98% (D), and 197% (F) increase in surface APP by the apoE monomer (NS: no significant), dimer, and trimer, respectively (n = 3, *p < 0.01). Levels of β-actin in the cell lysate were consistent across conditions (bottom panels).