Figure 3

Magnetofection does not affect motor neuron morphology and survival. Primary motor neurons (2 DIV) were transfected with Lifeact-GFP (A) or pmaxGFP (B) and fixed 1 day (A), or 3 and 5 days (B) after transfection. A. Motor neuron morphology was evaluated using the F-actin probe Lifeact-GFP and compared to rhodamine phalloidin staining. Cell body, dendrites (left) and growth cone (right) did not show any difference between transfected (top panel) and control (bottom) cells. Size bar: 10 μm. B. Surviving motor neurons were scored based on neurofilament (NF) and HB9 staining (left). Representative images are shown (bottom right). Bars represent mean and SEM from 3 independent experiments. One way repeated measures ANOVA, p > 0.05.