Visualization of BDNF mRNA with Htt and Ago2 proteins in vibratome sections of a P11 rat brain cortex. (a) A 3D projection of serial optical sections obtained by confocal microscopy. BDNF mRNA, Htt and Ago2 are shown in red, green and blue, respectively. Reconstruction method: LSM software, optical slice interval: 0.5 μm, stack size: 6.0 μm, N: nucleus, S: soma. White arrow indicates one co-localizing puncta of BDNF mRNA with Htt and Ago2. Scale bar: 5 μm. (b) Control FISH with a nick-translated probe generated from a control plasmid. DIC: differential interference contrast. Scale bar: 5 μm. (c) Percent co-localization of BDNF 3'UTR mRNA with Htt and Ago2. 16.6% and 18.1% of Htt was found to co-localize with BDNF mRNA and Ago2, respectively. 14.8% and 12.2% of BDNF mRNA was found to co-localize with Htt and Ago2, respectively.