Figure 1

Field Potential and Intracellular recording of CA1 pyramidal neurons after Wnt-5a treatment. (A, left), Superimposed, average (10 sweeps) of field potentials (fEPSPs) recorded from stratum radiatum of CA1 region before (a), after 30 min of Wnt-5a perfusion (b) and 20 min of washout (c). (A, right), Superimposed, normalized (to R1), averaged (10 sweeps) fEPSPs evoked by paired pulse stimulation (100 ms delay) in baseline and in presence of Wnt-5a (a+b). (A, middle), Time course of effect of Wnt-5a (black burbles) or Wnt-5a plus anti-Wnt-5a (white burbles) on fEPSPs peak amplitudes. Bottom, effect of Wnt-5a plus anti-Wnt-5a on fV. (B), Normalized amplitude of fEPSPs, evoked by the first stimulus (top), in control and after Wnt-5a or Wnt-5a plus anti-Wnt-5a treatment after 50 min of continued perfusion. Average values of normalized amplitude of fiber volley are shown (center), measured before (control) and after Wnt-5a (n = 6) or Wnt-5a plus Anti-Wnt-5a (n = 4) treatment application. Index of facilitation in both conditions (botton). (C, left), Superimposed, average EPSCs (20 sweeps) evoked by single stimulus and paired pulse stimulation at -90 and +40 mV of holding potential in control conditions (gray trace) and in the presence of Wnt-5a (black trace), respectively. (C, right) Summary data of average, normalized EPSCs amplitude and facilitation index, obtained in C in baseline and in the presence of Wnt-5a (n = 6), respectively. Bar represents the mean ± SEM (*p < 0.05 Student's t test).