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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Systemic treatment with liver X receptor agonists raises apolipoprotein E, cholesterol, and amyloid-β peptides in the cerebral spinal fluid of rats

Figure 2

CSF and soluble brain cholesterol were inversely altered by LXR agonist treatment. The levels of cholesterol in CSF and soluble brain extracts were evaluated using the Amplex Red Cholesterol Kit as described in Methods. A: Cholesterol levels in the CSF of rats treated with LXR agonists in comparison to the vehicle control. B: Cholesterol levels in the DEA-soluble brain fraction of rats treated with LXR agonists in comparison to the vehicle control. * p < 0.05 compared to vehicle treated controls by ANOVA, N = 4 per group. C: CSF samples were separated in native gel as described in Methods and then blotted with an anti-apoE antibody. Representative blot showing apoE immunoreactive bands in the CSF of rats treated with vehicle or T1317 for 3 days. CSF from vehicle-treated rats shows apoE-immunoreactive bands around 240 and 400 kD (←), while CSF from T1317-treated animals had strongest apoE-immunoreactivity in bands detected at approximately 400 and 650 kD (◀).

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Molecular Neurodegeneration

ISSN: 1750-1326