Figure 6
From: A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
N256S-kinesin-1A does not deplete neurofilaments from distal axons. Comparison of the neurofilament content of distal axons from cortical neurons expressing GFP-NFM alone (control) or GFP-NFM plus N256S-kinesin-1A. (A) Immunofluorescence microscopy for neurofilament protein (top panels) and for tubulin and actin (bottom panels; see Methods). Scale bar = 20 μm. (B) Quantification of neurofilament content in distal axons. There was no significant difference in the neurofilament content of axons expressing GFP-NFM plus N256S-kinesin-1A (49 axons) compared to control axons expressing GFP-NFM alone (49 axons; p = 0.73, Mann-Whitney test).