Figure 5
From: Age-related synaptic loss of the medial olivocochlear efferent innervation
Correlation between age-related loss of OHC and the MOC synapses. (A) One overlay of confocal images at 28 kHz from one 12-month-old YFP-12 mouse with the phalloidin staining for hair bundles (red) and YFP (green). Missing MOC synapse and OHCs were easily detected. (B) One overlay of confocal images at 10 kHz from one 12-month-old YFP-12 mouse with the phalloidin staining for hair bundles (red) and YFP (green). One missing OHC was observed. (C) These panels are from the same regions as (B). The top panel shows the layer of hair bundles and the appearance of OHC nuclei on the left side, which is closer to the lens than the right side. The middle and bottom panels show the MOC synapse at the location of one missing OHC.