The expression of GluR2 AMPA receptor in cultured cortical neurons. A: The expression of GluR2 in cortical neurons of DIV7 was examined by immunofluorescence analysis (right panel). The DIC image was shown for comparison (left panel). B: Cortical neurons were treated with amprolium (Amp, 0 or 1 mmol/L) for 1 or 4 days. The expression of GluR2 was examined by immunoblotting analysis. The expression of α-tubulin served as a loading control. C: The mRNA levels of GluR2 were determined by RT-PCR. The expression of β-actin mRNA served as an internal control. The experiments were replicated three times.