Figure 5
From: α-synuclein induced synapse damage is enhanced by amyloid-β1-42
Aβ 1-42 enhanced the αSN-induced inhibition of synaptic vesicle recycling. The amount of the fluorescent dye (FM1-43) taken up into synaptic vesicles in cortical neurons that had been incubated for 24 hours with different concentrations of αSN (●), or with αSN that had been premixed with either 7PA2-CM (■) or with CHO-CM (□) as shown and stimulated with ACh for 10 minutes. Values shown are the mean % fluorescence (where 100% = fluorescence in vehicle treated cortical neurons) ± SD, n = 18. (*) = Fluorescence significantly lower than in neurons incubated with the same concentration of αSN alone (P < 0.01).