Electron micrographs of cultures with and without drug treatment. (A-D) Exposure to SB (10 mM) for 36 hs resulted in accumulation of filamentous aggregates in differentiated 3D5 cells with induced α-Syn expression. (E-F) Without the SB treatment very few filamentous elements were detected in differentiated 3D5 cells with induced α-Syn expression. (B), (D) and (F) are higher magnification photographs of the framed area shown in (A), (C) and (E), respectively. The filaments have a diameter of either (B) 8-10 nm or (D) around 5 nm. Scale bar: 0.5 μm (A, C and E); 100 nm (B, D and F).