Skip to main content
Figure 1 | Molecular Neurodegeneration

Figure 1

From: Conformation dependent monoclonal antibodies distinguish different replicating strains or conformers of prefibrillar Aβ oligomers

Figure 1

Dot blot analysis of monoclonal antibody specificity. Nitrocellulose strips containing 1 ug spots of Aβ40 monomer, PFOs and fibrils and prefibrillar oligomers prepared from α-synuclein, immunoglobulin light chain, prion 106-126 peptide, KK(Q40)KK peptide, and calcitonin were probed with the monoclonal antibodies and with A11 polyclonal and 6E10 control antibodies. A11 stains all prefibrillar oligomers samples, while 6E10 recognizes only samples containing Aβ. The individual monoclonal antibodies only recognize Aβ PFOs and not Aβ monomer or fibril samples, but display distinct preferences for other types of PFOs. Mab204 displays the broadest reactivity, recognizing all PFOs except for light chain, while Mab 201 is the most restrictive reacting with only Aβ PFOs.

Back to article page

Molecular Neurodegeneration

ISSN: 1750-1326