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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Conformation dependent monoclonal antibodies distinguish different replicating strains or conformers of prefibrillar Aβ oligomers

Figure 5

Prefibrillar oligomers seed prefibrillar oligomer formation from monomer. A. The presence of a small amount of ladder like 10 - 56 kDa PFO seeds (prepared by dilution of NaOH stock solutions of Aβ40 into10 mM phosphate buffer pH 7.4), accelerates oligomer formation and gives rise to oligomers that stain with A11. No PFO immunoreactivity is observed in the non-seeded samples under these conditions. B. Mab 205 positive 35 - 45 kDa PFOs seed their own formation from Aβ40 monomer. C. Mab 205 35 - 45 kDa Aβ40 and Aβ42 PFOs seed the formation of A11 positive 35 - 45 kDa oligomers from Aβ42 monomers. Only the 35 - 45 kDa band is detected by A11, indicating that other sizes of oligomers are not formed. D. Seeding with PFOs suppresses the formation of fibrillar oligomers. In comparison, FOs spontaneously form in unseeded samples.

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Molecular Neurodegeneration

ISSN: 1750-1326