Colocalization of total PrPSc (rPrPSc + sPrPSc) and synaptophysin in synaptosomes. Confocal micrographs of a "crude" synaptosomal preparation obtained from the Sc237 prion strain infected hamsters at 70 dpi show total PrPSc (green) and synaptophysin (red) (mouse monoclonal anti-synaptophysin, clone SY38, DakoCytomation). Merged images show colocalization of total PrPSc and synaptophysin in yellow. Bar represents 20 μm. Adapted from Bouzamondo-Bernstein et al. .