Molecular Neurodegeneration

Table 1 Staining scores for cell cycle and DNA damage markers

From: DNA damage and cell cycle events implicate cerebellar dentate nucleus neurons as targets of Alzheimer's disease

Case Number	Diagnoses	Immunocytochemical score
		CycA	PCNA	Cdk4	53BP1	pATM
1	AD	0	1	1	2	-
2	AD	1	-	2	1	-
3	AD	3	2	2	5	4
4	AD	0	0	5	0	0
5	AD	1	0	2	0	5
6	AD	1	0	4	0	3
7	AD	0	1	5	0	3
8	AD	1	1	2	5	4
9	AD	1	1	1	0	-
10	AD	2	0	0	4	5
11	AD	0	1	2	4	5
12	AD	1	0	2	5	5
13	AD	3	3	5	0	5
14	AD	0	1	1	3	5
15	AD	0	1	0	5	-
16	AD	1	1	-	0	-
17	AD	0	0	-	2	-
18	AD	3	3	5	2	0
19	AD	1	0	5	4	5
20	AD	4	3	1	1	1
21	AD	2	1	4	1	3
22	AD	0	0	4	0	5
23	Con	0	0	0	0	0
24	Con	0	0	0	1	0
25	Con	0	2	0	2	0
26	Con	0	2	0	0	0
27	Con	0	0	0	0	0
28	Con	0	1	3	0	4
29	Con	0	0	2	0	-
30	Con	0	0	0	1	0
31	Con	0	0	0	0	0
32	Con	0	0	0	0	0
33	Con	0	0	0	0	0
34	Con	0	0	2	0	0
35	Con	-	2	0	0	0
36	Con	0	3	-	0	0
37	Con	0	2	-	0	0
38	Con	0	2	-	0	0
39	Con	0	2	-	0	0
40	Con	1	1	1	0	0
41	Con	0	1	3	2	0
P value t-test (2-tailed)		CycA	PCNA	Cdk4	53BP1	P-ATM
		0.0004	1.0	0.0005	0.001	0.000001

Each sample was scored based on % of CDN neurons stained and the staining intensity. 0 = no staining; 1 = very few cells stained or weakly stained; 2 = moderate staining, 5-10% of the CDN neurons; 3 = 10-25% moderately to strongly stained; 4 = more than 25% strongly stained; 5 = more than 50% strongly stained. The p-values were calculated with Student's t-test, 2-tailed.

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ISSN: 1750-1326

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