Figure 1
From: Identification of BACE1 cleavage sites in human voltage-gated sodium channel beta 2 subunit
Identification of BACE1 cleavage sites in human Navβ2 by MS analysis. Reaction mixtures from an in vitro BACE1 cleavage assay were resolved on a 12% BisTris gel and full-length peptide and cleavage products were detected by silver staining. Only the incubation of the β2-peptide with BACE1 generates a ~2 kDa cleavage product. GL-189, a BACE1 inhibitor, significantly decreased the amount of cleavage product (lane 3). B) Western blot analysis of an in vitro BACE1 cleavage assay samples using anti-biotin-HRP conjugate. C) Table containing the major cleavage products and their predicted sequences determined by Mass spectrometry from control and the BACE1 reaction sample. D) Sequence of the β2-peptide synthesized for the in vitro cleavage assay. A biotinylated tyrosine amino acid is added to the N-terminus of the peptide for detection by anti-biotin-HRP conjugate. One major (big arrow) and one minor (small arrow) BACE1 cleavage site were detected by MS analysis.