Figure 4
From: Identification of BACE1 cleavage sites in human voltage-gated sodium channel beta 2 subunit
Mutations in the BACE1 cleavage site strongly decrease processing of Navβ2 in B104 cells overexpressing BACE1. A) B104 cells stably overexpressing BACE1 were transfected with Navβ2 wild-type, Navβ2 (147ML/VI) or Navβ2 (147ML/AA) and then subjected to Western blot analysis. B) Quantification of Navβ2-CTF to Navβ2-FL ratios from A. Navβ2-CTF levels were decreased by mutations in the BACE1 cleavage site (FL, full length; CTF, C-terminal fragment).