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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Protein S blocks the extrinsic apoptotic cascade in tissue plasminogen activator/N-methyl D-aspartate-treated neurons via Tyro3-Akt-FKHRL1 signaling pathway

Figure 2

PS protects tPA/NMDA-treated neurons via activation of PI3K/Akt signaling. (A) Western blots for pAkt (Ser473) and total Akt in whole-cell extracts from mouse cortical neurons 2 h after tPA/NMDA exposure in the presence or absence of PS and LY294002 (50 μM). (B) Intensity of pAkt signal was measured by scanning densitometry and normalized to total Akt. (C) Akt activity was determined 2 h after tPA/NMDA exposure in the presence or absence of PS using an immune-complex kinase assay with GSK3 as the substrate. (D) Intensity of the pGSK3 signal was normalized to β-actin. (E) Survival of mouse cortical neurons 24 h after tPA/NMDA exposure in the presence or absence of PS and LY294002 (50 μM). LY294002 was added to the culture 1 h before tPA/NMDA treatment. In all studies, murine PS was used at 100 nM and was added simultaneously with tPA/NMDA. Mean ± SEM, n = 3 independent cultures in triplicate. NS, non-significant.

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Molecular Neurodegeneration

ISSN: 1750-1326