Low NO concentrations suppress BACE1 transcription, which is likely mediated through cGMP-PKG-upregulated PGC-1α. (A) Effect of nitrosative stress on BACE1 mRNA levels was examined by qRT-PCR. Lower concentrations of SNOC inhibited while higher concentrations of SNOC had no effect on BACE1 transcription. (B) Effect of cGMP inhibitor, 1H-[1.2.4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODG, Sigma) in BACE1 transcription. (C) Effect of NO on PGC-1α mRNA levels. (D) Effect of PGC-1α overexpression on the promoter activity BACE1 from both human and rat origins, as assessed by luciferase assay with/without transfection of PGC-1α. (E) Effect of PGC-1α on BACE1 mRNA levels was assessed with qRT-PCR.