Figure 8

p53 is required for 6-OHDA-induced cell death. Primary cultures from individual pups from p53 +/- ×p53 +/- matings were treated with 20 μM 6-OHDA. A) After 24 hours, cultures were fixed and stained for TH. B) TH-positive neurons were counted and survival was expressed as a percent of untreated control. Data were analyzed by one-way ANOVA (***, p < 0.001) with Bonferroni post-tests to compare to wild-type (-/-, **, p < 0.01). C) TH-positive neurite length was estimated using unbiased stereology and expressed as a percent of untreated control. Data were analyzed by Student's t-test (*, p < 0.05). D) Total RNA was isolated at the indicated times and Puma and 18S rRNA RNA levels were analyzed by semi-quantitative RT-PCR. Data were analyzed by two-way ANOVA (treatment: **, p < 0.01; genotype: *, p < 0.05). E) Total RNA was isolated at the indicated times and CHOP and GAPDH RNA levels were analyzed by qPCR. Data were analyzed by two-way ANOVA (treatment: ***, p < 0.001; genotype: ns). F) Cells treated for 24 hours were co-stained for TH and CHOP. TH-positive and CHOP-positive cells were counted and the percentage of TH-positive cells that were also CHOP-positive was calculated. Data were analyzed by two-way ANOVA (treatment: ***, p < 0.001; genotype: ns). 11