Disruption of GLUT1 affects the integrity of BBB. (A-D) Immunohistochemistry of occludin in intact brain microvessels of control (A), METH (15 mg/kg body weight) (B), CB (20 μM) (C) and METH+ALC (ALC = 200 μM) (D) treated mice. (E) Western blot analysis of tight junction proteins, occludin (a 65 kDa protein) and (F) ZO-1 (225 kDa protein) in hBECs. Bar graphs show the results that are expressed as ratio of occludin or ZO-1 to that of α-actin bands and presented as the mean values (± SEM; n = 4). Scale bar indicates 20 μm in A-D.