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Table 2 DeCyder MS analysis for ion trap MS/MS of recombinant parkin exposed to H2O2

From: Oxidation of the cysteine-rich regions of parkin perturbs its E3 ligase activity and contributes to protein aggregation

Domain (Residue #) Peptide Sequence Observed (m/z) Monoiostopioc M r Calculated Observed Ratio
RING I 246SPVLVFQC(SO2H)NSR256 640.912+ 1280.62 1279.81 n/a
(238 - 293) 246SPVLVFQC(SO3H)NSR256 649.422+ 1296.61 1296.83 n/a
IBR 349KVTCEGGNGLGC(SO3H)GFAFCR366 623.313+ 1865.79 1866.91 3.53
(294 - 417) 350VTC(SO2H)EGGNGLGC(SO3H)GFAFCREC(SO2H)K369 721.513+ 2161.82 2161.50 n/a
RING II 421C(SO3H)HVPVEK427 421.932+ 842.40 841.84 13.15
(418 - 449) 428NGGC(SO2H)MHM(Oxidation)KCPQPQC(CAM)R442 897.892+ 1793.69 1793.77 n/a
Others 90NAAGGC(SO3H)EREPQSLTR104 819.022+ 1635.73 1636.03 5.20
(1 - 237) 212C(SO3H)GAHPTSDKETPVALHLIATNSR234 822.403+ 2465.20 2464.17 1.65