Figure 6

overburdened aggregation of MAP1b-LC in the DJ-1 KD SH-SY5Y cells leads to apoptosis. A, pcNDA3-MAP1b-LC or blank pcDNA3 were transfected into DJ-1 KD SH-SY5Y cells or scrambled controls. pDsRed2 was co-transfected to mark the transfected cells. Sixty hours later, cells were stained by Annexin-v and analyzed by flow cytometer. The data showed that MAP1b-LC can induce apoptosis (23% Annexin-v positive of the total transfected cells); the apoptosis cells increased to 35% in the DJ-1 KD cells compared to the scramble. B, The statistical analysis result showed that the apoptosis was increased when MAP1b-LC was introduced into DJ-1 KD SH-SY5Y cells (*, p = 0.04)