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Table 1 Primers designed and used for IGF-1, leptin, IGF-1 promoter and leptin promoter

From: Molecular interplay between leptin, insulin-like growth factor-1, and β-amyloid in organotypic slices from rabbit hippocampus

GENE PRIMER GenBank Accession Number Sequence  
IGF-1 Forward AF022961 5'-AGGCTATGGCTCCAGCATTCG-3' RT-PCR
IGF-1 Reverse AF022961 5'-AGTCTTGGGCATGTCAGTGTGG-3' RT-PCR
Leptin Forward AF203903 5'-AGTCTTGGGCATGTCAGTGTGG-3' RT-PCR
Leptin Reverse AF203903 5'-AGTCTGCCGTCCCGAAATGTG-3' RT-PCR
IGF-1 promoter Site-1 AC010202 5'-CCAGGGTCTCCAAGCCACTG-3' EMSA
IGF-1 promoter Site-2 AC010202 5'-AAATTCTAAGAAACT-3' EMSA
IGF-1 promoter Site-1 Forward AC010202 5'-TTTTTCTTAGAAGTA-3' ChIP
IGF-1 promoter Site-1 Reverse AC010202 5'-GATTGGTTGTGTGGCATGAG-3' ChIP
IGF-1 promoter Site-2 Forward AC010202 5'-TGGCATGTTTTGAGGTTTTG-3' ChIP
IGF-1 promoter Site-2 Reverse AC010202 5'-ACAAGCCCACGGGGTATGGC-3' ChIP
Leptin promoter Site 1 Forward NC013675 5'-CTTCTGAGCCTTGGGCATGTCG-3' ChIP
Leptin promoter Site 1 Reverse NC013675 5'-ACACACAACACCTGCCAAAA-3' ChIP
Leptin promoter Site 2 Forward NC013675 5'-CACAGCACTAGGTCCAGCAG-3' ChIP
Leptin promoter Site 2 Reverse NC013675 5'-ATGTGGAGTGACCCGAGAGT-3' ChIP