Table 1 Primers designed and used for IGF-1, leptin, IGF-1 promoter and leptin promoter
GENE | PRIMER | GenBank Accession Number | Sequence | |
|---|---|---|---|---|
IGF-1 | Forward | AF022961 | 5'-AGGCTATGGCTCCAGCATTCG-3' | RT-PCR |
IGF-1 | Reverse | AF022961 | 5'-AGTCTTGGGCATGTCAGTGTGG-3' | RT-PCR |
Leptin | Forward | AF203903 | 5'-AGTCTTGGGCATGTCAGTGTGG-3' | RT-PCR |
Leptin | Reverse | AF203903 | 5'-AGTCTGCCGTCCCGAAATGTG-3' | RT-PCR |
IGF-1 promoter | Site-1 | AC010202 | 5'-CCAGGGTCTCCAAGCCACTG-3' | EMSA |
IGF-1 promoter | Site-2 | AC010202 | 5'-AAATTCTAAGAAACT-3' | EMSA |
IGF-1 promoter | Site-1 Forward | AC010202 | 5'-TTTTTCTTAGAAGTA-3' | ChIP |
IGF-1 promoter | Site-1 Reverse | AC010202 | 5'-GATTGGTTGTGTGGCATGAG-3' | ChIP |
IGF-1 promoter | Site-2 Forward | AC010202 | 5'-TGGCATGTTTTGAGGTTTTG-3' | ChIP |
IGF-1 promoter | Site-2 Reverse | AC010202 | 5'-ACAAGCCCACGGGGTATGGC-3' | ChIP |
Leptin promoter | Site 1 Forward | NC013675 | 5'-CTTCTGAGCCTTGGGCATGTCG-3' | ChIP |
Leptin promoter | Site 1 Reverse | NC013675 | 5'-ACACACAACACCTGCCAAAA-3' | ChIP |
Leptin promoter | Site 2 Forward | NC013675 | 5'-CACAGCACTAGGTCCAGCAG-3' | ChIP |
Leptin promoter | Site 2 Reverse | NC013675 | 5'-ATGTGGAGTGACCCGAGAGT-3' | ChIP |