Activated PKD1 translocates to nucleus during H
-induced oxidative stress. Immunofluorescence analysis of N27 dopaminergic cells stained for activated PKD1 using fluorescence microscopy during H2O2 exposure show translocation to nucleus. PKD1pS744/S748 - Green, Nucleus - Blue. Nuclei were stained with Hoechst dye (A). Immunofluorescence analysis of primary dopaminergic neurons staining for TH obtained from the mouse substantia nigral region shows translocation of activated PKD1 to the nucleus during H2O2 exposure.TH-Green, PKD1pS744/S748 - Red, Nucleus - Blue, Merge - Pink. Nuclei were stained with Hoechst dye (B). Primary dopaminergic neurons staining for TH show presence of PKD1pS916 in both cytosol and nucleus during H2O2 exposure. TH - Green, PKD1pS916- Red, Nucleus - Blue, Pink - Merge in nucleus, yellow -Merge in cytosol. Nuclei were stained with Hoechst dye (C).