Relative quantification of ERV-expression in BSE-infected versus non-infected macaques using QPCR. Quantitative expression of ERV-E4-1 and MacERV-4 were assessed by QPCR. Expression was normalized to the housekeeping gene RPII. Points represent the mean of two experiments in duplicates is shown. Statistical significance was determined by unpaired students t-test (*** p < 0.001; ** p < 0.01; * p < 0.05).