Figure 8

Aβ-related cell surface receptors phenotype changes in microglial cells. BV-2 cells were pre-stimulated with oAβ(1-42) (0, 1.0 and 5.0 μM) for 12 h or IL-1β (20 ng/ml) for 18 h or LPS (1.0 μg/ml) and or t-BHP (100 μM) for 1 h, then followed by an addition of incubation with fAβ(1-42) (5.0 μM) for 30 min. Total RNA was extracted, and mRNA expression of Aβ-related microglial cell surface receptors including CD36, CD47, integrin β1 (Itgb1), SRA, SRB1, RAGE, FPR2, FcγR I and FcγRIII, were analyzed by real-time PCR(A-I). All gene expressions were normalized to GAPDH level. The level of control expression was fixed at 1.0. Data were expressed as relative folds compared to control. Values correspond to mean ± S.E (n = 3). *P < 0.05, **P < 0.01 or ***P < 0.001 compared with the control; ^#P < 0.05,^##P < 0.01 or ^###P < 0.001, compared with the fAβ(1-42) alone.