Oligodendrocyte (OL) survival and oligodendrocyte progenitor proliferation in the white matter of ex vivo slice culture model. (A) Percentage of total OL lineage cells in the white matter that labeled with cleaved caspase-3+ (CC3). Data are mean ± SEM (n = 3-4 slices per time point). (B) Example of CC3+ (red, arrows) and Olig2+ (green, arrowheads) cells (co-localized cells are shown in yellow). (C) Density of PDGFRα+ (black bars, left axis) and co-localized PDGFRα+/Ki67+ OPCs in the white matter. Data are mean ± SEM (n = 6 slices per time point from two independent experiments). *P < 0.01 (ANOVA followed by Tukey's multiple comparison test; uncorrected data). †P < 0.01 (ANCOVA followed by Tukey's multiple comparison test; data corrected for atrophy). (D-F) PDGFRα+ OPCs in the white matter at 0 DIV (D), 1 DIV (E) and 5 DIV (F). The inset in E shows a PDGFRα+ (green)/Ki67+ (red) double labeled OPC. Scale: B, D-F, 20 μm; E inset, 10 μm.