Figure 3

Effects of compound-23 on oxidative stress-induced death of rat mesencephalic neurons. (A) Rat mesencephalic cultured neurons were fixed and immunostained by anti-NeuN and anti-TH antibodies. Cells were then stained with DAPI. The cells were then reacted with a rhodamine-conjugated anti-rabbit IgG or fluorescein isothiocyanate-conjugated anti-mouse IgG and observed under an All-in-on microscope. (B) Rat mesencephalic cultured neurons were treated with 1 μM of comp-23 for 20 hours and with 200 μM H₂O₂ for 3 hours, and cell viability was measured by an MTT assay. Significance: **P < 0.01 versus vehicle (DMSO) control without compound.