Treatment of SH-SY5Y neurons induces co-localization of TDP-43 and ubiquitin in SGs. Cells were treated overnight with 1 mM paraquat and localization of TDP-43 and ubiquitin was determined by immunofluorescence. A-D: untreated, E-L: paraquat treated. Green = TDP-43, red = ubiquitin, blue = DAPI. D, H and L represent merged images of panels above. Solid arrows indicate co-localization of TDP-43 and ubiquitin in SGs. Arrowhead indicates lack of co-localization of TDP-43 and ubiquitin in one SG in panel F. Dotted arrow indicates lack of co-localization of diffuse TDP-43 and ubiquitin. Bar = 10 μm. Representative images from three separate experiments performed in duplicate or triplicate.