Astrogliosis caused by the presence of mutant huntingtin induces preservation of striatal BDNF protein levels in R6/2:pGFAP-BDNF mice. Striatal GFAP immuno-reactivity in 12-week-old wt, pGFAP-BDNF, R6/2 and R6/2:pGFAP-BDNF mice (A). The levels of GFAP (B) and mature BDNF (C) were analyzed by Western blot of protein extracts obtained from striata of 12-week old wt, pGFAP-BDNF, R6/2 and R6/2:pGFAP-BDNF mice. Representative immunoblots are also shown (B-C). Note loading control is different in each blot due to the huge difference on molecular weight of GFAP and mature BDNF and different blot characteristics. Values are expressed as mean ± s.e.m. Data were analyzed by one-way ANOVA: * p < 0.05 and *** p < 0.001, compared with the wt genotype; $ p < 0.05 and $$$ p < 0.001, compared with the pGFAP-BDNF genotype; and # p < 0.05, compared with the R6/2 genotype (n = 7-12/genotype). Scale bar 50 μm.