The down-regulation of DARPP-32 and enkephalin protein levels in R6/2 mice is improved in R6/2:pGFAP-BDNF mice. The levels of enkephalin (A) and DARPP-32 (B) were analyzed by Western blot of protein extracts obtained from striatum of 12-week-old wt, pGFAP-BDNF, R6/2 and R6/2:pGFAP-BDNF mice. Representative immunoblots are shown. Values are expressed as mean ± s.e.m. Data were analyzed by one-way ANOVA. ** p < 0.01 and *** p < 0.001, as compared to wt mice; $ p < 0.05, $$ p < 0.01 and $$$ p < 0.001, as compared to pGFAP-BDNF mice; and # p < 0.05, as compared to R6/2 mice (n = 7-12/genotype).