Further characterization of the intracellular localization of DsRed in neurons Thy1-mitoDsRed2 tg mice. Immunofluorescent localization of catalase (A), LC3A (B) and cytochrome P450 reductase (C), as markers for peroxisomes/nucleus, autophagic vesicles and endoplasmic reticulum (ER), respectively, in brainstem sections shows mitochondrial-specific labeling with DsRed. Cell nuclei (blue) were visualized with the DNA stain Hoechst-33342 dye. A. Large brainstem neurons showing intracellular segregation of catalase immunoreactivity (green) and DsRed. Scale bar = 7 μm. B. Brainstem neurons showing partial overlap of cytoplasmic DsRed and LC3A (green) seen as yellow. Individual red and green signals are also seen. Scale bar = 5 μm. C. Brainstem locus ceruleus neurons (red, right) peri-cerulean neurons showing distinct DsRed and ER (green) intracellular localizations. Scale bar = 5 μm. Images are representative of several different tg mice (n = 8).