The SEDI antibody recognizes immobile punctate structures in cells expressing A4V hSOD1. HEK293FT cells were transiently transfected with expression plasmids for either WT (A-D) or A4V (E-L) hSOD1 for 24 hours and stained with the SEDI or C4F6 antibody as explained in Methods. Pictures were taken with a 40x objective. Scale bars = 10 μm. The images in I-L were digitally magnified from A4V SOD1 cells stained with SEDI/DAPI. The images shown are representative of at least 3 independent transfection experiments.