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Figure 12 | Molecular Neurodegeneration

Figure 12

From: Mitosis-specific phosphorylation of amyloid precursor protein at Threonine 668 leads to its altered processing and association with centrosomes

Figure 12

P-APP co-localization with MPM-2 at centrosomes in metaphase cells: Asynchronously growing (untreated, top row) and nocodazole arrested (bottom row) H4-15X cells were immunostained using the mitosis specific monoclonal antibody MPM2 and Thr668 P-APP polyclonal antibodies and staining was visualized using Alexa 488 and 594 fluorophores respectively. The untreated cells show P-APP localization in centrosomes in the mitotic cells. In the cells arrested with nocodazole the microtubules were completely depolymerized and P-APP showed significantly higher levels of amorphous staining. The nuclei were visualized using Hoechst stain. Magnification: 63×.

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Molecular Neurodegeneration

ISSN: 1750-1326