Figure 4

SEPT11 immunoreactivity in control and FTLD-U using polyclonal N-terminal antibody. (a-b) Double label immunofluorescence in control (a) and FTLD-U (b) frontal cortex reveals overlap between SEPT11 (red) and glial fibrillary acidic protein (GFAP; green), suggesting that cell-associated staining localizes to astrocyte cell bodies and processes. (c-e) IHC in FTLD-U (d and e) frontal cortex detects both linear and rounded accumulations of SEPT11 that are absent in control (c). Unlike in (a) and (b), these SEPT11 threads were not associated with specific cell bodies. (f) Summary of blinded scoring of frontal cortex from 81 cases and controls for the presence of SEPT11 threads.