Figure 1

Generation of Hup23 mice. A, Schematic representation of the transgenic construct. Human p23 was expressed under the transcriptional control of mouse Thy-1.2 promoter using Thy-1.2 genomic expression cassette. B, Genotyping of Hup23 mice. Genotyping was performed by PCR of tail DNA using primers specific to human p23 cDNA (upper panel) to amplify a 466-bp PCR product. Control reactions were performed using primers specific for mouse Tmed10 locus (lower panel) to amplify 712-bp PCR product. C, The distribution of endogenous and transgene-derived p23. Sagittal brain sections and cross sections of spinal cord from Ntg and Hup23 mice were stained by immunoperoxidase or immunofluorescence labeling, respectively, using polyclonal p23 antibody. In agreement with the previously described pattern of transgene expression from the Thy-1.2 genomic expression cassette [15–17], p23 is broadly overexpressed in brain and spinal cord. Brainstem (Bs), caudate putamen (CP), cerebellum (Cb), cortex (Cx), hippocampus (H), hypothalamus (Hy), midbrain (Mb), substantia nigra (SN), thalamus (Th), olfactory bulb (OB), and ventral striatum (VS) are indicated in sagittal brain section. Spinal motoneurons (MNs), lateral spinal nucleus (LSN), rubrospinal tract (RS), lateral funiculus (LF), ventral funiculus (VF), and ventral horn of gray matter (VH) are indicated in spinal cord sections.