Figure 6

Analysis of p23 and synaptic marker expression. Double immunofluorescence analysis was performed on sagittal brain sections stained with indicated antibodies using confocal microscopy. A, Pyramidal neurons in frontal cortex of Hup23 mice show overexpression of p23 (green) and similar presynaptic distribution of synaptophysin (red). B, Synaptophysin staining in hippocampal CA1 region, cerebellum, and brainstem appears indistinguishable between Hup23 and Ntg mice. C, Normal appearance of Purkinje cells visualized by calbindin staining, as well as VGLUT1 and VGLUT-2 labeling of parallel fibers and climbing fibers, respectively, in cerebellum of Hup23 animals. The following areas are indicated: Pcl, Purkinje cell layer; gcl, granule cell layer, ml, molecular layer; sp, stratum pyramidale; sr, stratum radiatum.