Figure 3

The locomotive ability affected by the expression of different forms of Fus or Caz. (A) Expression of Fus/Caz in fly motor neurons impairs the locomotive ability in larvae. A graph of the average moving distance of the third instar larvae traveling over 30 sec is shown here (n = 15 male larvae scored for each group). Each larva was tested 3 times. The OK371-Gal4 was used for these experiments. (B) Larvae brains with VNCs from experiments in A were subjected to Western blots with antibodies against the epitope tags to monitor the comparable levels of protein expression. GFP serves as an internal protein expression and loading control. (C-D) Graphs of average climbing distance of the adult flies at Day 2 or Day 10 after emergence. Each adult fly was placed at the bottom of a 2 ml pipette and allowed to climb vertically for 30 s (n = 20 male flies for each group). Each fly was counted 3 times. The height that the flies climbed on the inside wall of the pipette is an indication of their locomotion ability. The D42-Gal4 was used for these experiments. The "#" symbol indicates pupal lethality. The larval locomotion was significantly impaired by Fus, FusR521G, and Caz expression, but not by FusΔ32 expression.