Figure 5
13C 6 -leucine brain Aβ labeling in the presence of ABCA1 overexpression and deletion. (A) Cohorts of wildtype and ABCA1 transgenic mice and (C) wildtype and ABCA1−/− mice were labeled with 13 C6-leucine and the brains isolated after predetermined time points. Total Aβ was immunoprecipitated from the cortex and the labeled/unlabeled ratios were then plotted versus time for each genotype. For FCR measurements, the data was plotted as in 3B and the monoexponential slopes were calculated for both (B) ABCA1 Tg and (D) ABCA1−/− mice and their respective Wt controls (n = 5-6 mice per time point, error bars represent SEM, dotted lines represent 95% confidence band).